Anti-HSP90B1 Polyclonal Antibody

Catalog No.

PHD05601

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSP90B1 (Asp22-Ala311).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

94 kDa glucose-regulated protein,HSP90B1,gp96 homolog,GRP94,Heat shock protein 90 kDa beta member 1,Endoplasmin,TRA1,Tumor rejection antigen 1,GRP-94

Purification

Purified by antigen affinity column.

Accession

P14625

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Functional epitope mapping of cell surface glucose-regulated protein 94: A combinatorial approach for therapeutic targeting., PMID:39880265

Targeting cell surface glucose-regulated protein 94 in gastric cancer with an anti-GRP94 human monoclonal antibody., PMID:38449302

Transcriptomic Analysis Reveals Sixteen Potential Genes Associated with the Successful Differentiation of Antibody-Secreting Cells through the Utilization of Unfolded Protein Response Mechanisms in Robust Responders to the Influenza Vaccine., PMID:38400120

Single-cell transcriptomic atlas throughout anti-BCMA CAR-T therapy in patients with multiple myeloma., PMID:38035111

MZB1-expressing cells are essential for local immunoglobulin production in chronic rhinosinusitis with nasal polyps., PMID:37852603

HSP90B1-mediated plasma membrane localization of GLUT1 promotes radioresistance of glioblastomas., PMID:37750323

Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection., PMID:37434783

An internalizing antibody targeting of cell surface GRP94 effectively suppresses tumor angiogenesis of colorectal cancer., PMID:35658213

Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells., PMID:35123066

Proteomic changes of aqueous humor in proliferative diabetic retinopathy patients treated with different intravitreal anti-VEGF agents., PMID:35032522

Lrp1 is a host entry factor for Rift Valley fever virus., PMID:34559985

Broadly Protective CD8+ T Cell Immunity to Highly Conserved Epitopes Elicited by Heat Shock Protein gp96-Adjuvanted Influenza Monovalent Split Vaccine., PMID:33827939

Cell Surface GRP94 as a Novel Emerging Therapeutic Target for Monoclonal Antibody Cancer Therapy., PMID:33802964

Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice., PMID:33584668

gp96 Is Critical for both Human Herpesvirus 6A (HHV-6A) and HHV-6B Infections., PMID:32295911

Endoplasmic reticulum stress promotes inflammation-mediated proteolytic activity at the ocular surface., PMID:32042069

Glycoprotein 96 polymorphisms are associated with the risk of systemic lupus erythematosus: A case-control study., PMID:30860673

Improving the potency of DNA vaccine encoding HIV-1 Nef antigen using two endogenous adjuvants in mouse model., PMID:29061065

Gut homeostasis and regulatory T cell induction depend on molecular chaperone gp96 in CD11c+ cells., PMID:28526855

Immuno-Stimulating Peptide Derived from HMGB1 is More Effective Than the N-Terminal Domain of Gp96 as an Endogenous Adjuvant for Improvement of Protein Vaccines., PMID:28000571

Transformation of the Non-Selective Aminocyclohexanol-Based Hsp90 Inhibitor into a Grp94-Seletive Scaffold., PMID:27959508

Primary Human and Rat β-Cells Release the Intracellular Autoantigens GAD65, IA-2, and Proinsulin in Exosomes Together With Cytokine-Induced Enhancers of Immunity., PMID:27872147

Grp94 in complexes with IgG is a soluble diagnostic marker of gastrointestinal tumors and displays immune-stimulating activity on peripheral blood immune cells., PMID:27662661

Heat-Shock Protein gp96 Enhances T Cell Responses and Protective Potential to Bacillus Calmette-Guérin Vaccine., PMID:27417661

Gp96-Ig/Costimulator (OX40L, ICOSL, or 4-1BBL) Combination Vaccine Improves T-cell Priming and Enhances Immunity, Memory, and Tumor Elimination., PMID:27364122

Development of Glucose Regulated Protein 94-Selective Inhibitors Based on the BnIm and Radamide Scaffold., PMID:27003516

GRP94/gp96 in Cancer: Biology, Structure, Immunology, and Drug Development., PMID:26916005

Autoantibodies to Heat-Shock Protein, HSPA5, and Epitope Spreading: High-Dose Dexamethasone Therapy Rescues Ovarian Function in Experimental Autoimmune Ovarian Insufficiency Mouse Model., PMID:26840828

Chaperome screening leads to identification of Grp94/Gp96 and FKBP4/52 as modulators of the α-synuclein-elicited immune response., PMID:26443817

Chaperone gp96 mediates ER-α36 cell membrane expression., PMID:26396174

Proteomic Investigation on Grp94-IgG Complexes Circulating in Plasma of Type 1 Diabetic Subjects., PMID:26167512

Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer., PMID:25546612

Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers., PMID:25225353

[Expression of anti-gp96 scFv fragment in Pichia pastoris and identification of its biological activity]., PMID:25195249

Reproducibility and efficiency of serum-derived exosome extraction methods., PMID:24956264

Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings., PMID:24891284

MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress., PMID:24888588

Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells., PMID:24880126

Activation of anti-HBV immune activity by DNA vaccine via electroporation using heat shock proteins as adjuvant., PMID:24660624

Heat shock proteins purified from autologous tumors using antibody-based affinity chromatography., PMID:24619689

Structural insights into complexes of glucose-regulated Protein94 (Grp94) with human immunoglobulin G. relevance for Grp94-IgG complexes that form in vivo in pathological conditions., PMID:24489700

[Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells]., PMID:24343717

Napyradiomycins CNQ525.510B and A80915C target the Hsp90 paralogue Grp94., PMID:24292715

Secreted heat shock protein gp96-Ig: next-generation vaccines for cancer and infectious diseases., PMID:24254084

Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues., PMID:23602083

Essential roles of grp94 in gut homeostasis via chaperoning canonical Wnt pathway., PMID:23572575

Isolation and identification of renal cell carcinoma-derived peptides associated with GP96., PMID:23448575

Cutting edge: novel vaccination modality provides significant protection against mucosal infection by highly pathogenic simian immunodeficiency virus., PMID:23401588

Listeria monocytogenes triggers the cell surface expression of Gp96 protein and interacts with its N terminus to support cellular infection., PMID:23109341

Glycoprotein 96 perpetuates the persistent inflammation of rheumatoid arthritis., PMID:22777994

Document Download

Anti-HSP90B1 Polyclonal Antibody.pdf