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Anti-MYH1 Polyclonal Antibody

Catalog #:   PHC95501 Specific References (10) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P12882
Overview

Catalog No.

PHC95501

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MYH1 (Pro842-Lys967).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Myosin heavy chain 2x,Myosin heavy chain 1,Myosin-1,MyHC-IIx/d,MyHC-2x,Myosin heavy chain,skeletal muscle,adult 1,Myosin heavy chain IIx/d,MYH1

Purification

Purified by antigen affinity column.

Accession

P12882

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MYH1 antibody (PHC95501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 17 kDa
    Observed MW: 17 kDa
  • Immunocytochemistry/ Immunofluorescence
    MYH1 in HaCat Cell Line.
    The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with MYH1 (PHC95501) at 9.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Protein profile of fiber types in human skeletal muscle: a single-fiber proteomics study., PMID:34727990

Quantification of biomarkers for beef meat qualities using a combination of Parallel Reaction Monitoring- and antibody-based proteomics., PMID:32078991

Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining., PMID:28752107

Exploratory plasma proteomic analysis in a randomized crossover trial of aspirin among healthy men and women., PMID:28542447

A role for Myh1 in DNA repair after treatment with strand-breaking and crosslinking chemotherapeutic agents., PMID:23677513

Myostatin facilitates slow and inhibits fast myosin heavy chain expression during myogenic differentiation., PMID:22910409

Myogenic capacity of muscle progenitor cells from head and limb muscles., PMID:22288919

Impact of static magnetic fields on human myoblast cell cultures., PMID:21837362

Characterization of human myoblast differentiation for tissue-engineering purposes by quantitative gene expression analysis., PMID:21370490

An altered phenotype in a conditional knockout of Pitx2 in extraocular muscle., PMID:19407022

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-MYH1 Polyclonal Antibody [PHC95501]
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