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Anti-HSPA1A Polyclonal Antibody

Catalog #:   PHC69301 Specific References (29) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P0DMV8
Overview

Catalog No.

PHC69301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA1A (Met1-Asp641).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HSP70.1,HSP70-1,HSPA1,HSPA1A,Heat shock 70 kDa protein 1A,Heat shock 70 kDa protein 1,HSP72,HSX70

Purification

Purified by antigen affinity column.

Accession

P0DMV8

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPA1A antibody (PHC69301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 72 kDa
    Observed MW: 72 kDa
References

Single-cell transcriptomic analysis reveals distinct plasma cell populations in chronic thromboembolic pulmonary hypertension., PMID:39965671

Comprehensive multiplexed autoantibody profiling of patients with advanced urothelial cancer., PMID:38309723

Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection., PMID:37434783

Integrative system biology and mathematical modeling of genetic networks identifies shared biomarkers for obesity and diabetes., PMID:35240786

FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress., PMID:34239032

Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells., PMID:32276468

Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment., PMID:31588238

HSP70 Facilitates Memory Consolidation of Fear Conditioning through MAPK Pathway in the Hippocampus., PMID:29374537

Overexpression of cytosolic, plasma membrane bound and extracellular heat shock protein 70 (Hsp70) in primary glioblastomas., PMID:28849427

Molecular markers of systemic autoimmune disorders: the expression of MHC-located HSP70 genes is significantly associated with autoimmunity development., PMID:28032847

Systematic protein-protein interaction and pathway analyses in the idiopathic inflammatory myopathies., PMID:27388770

[Role of heat shock proteins in the cardioprotection of regular moderate alcohol consumption]., PMID:26902796

Hsp70 (HSPA1) Lysine Methylation Status as a Potential Prognostic Factor in Metastatic High-Grade Serous Carcinoma., PMID:26448330

Early inflammatory response in polytraumatized patients: Cytokines and heat shock proteins. A pilot study., PMID:26068807

Reactive γ-ketoaldehydes promote protein misfolding and preamyloid oligomer formation in rapidly-activated atrial cells., PMID:25463275

Transcriptome profiling and genome-wide DNA binding define the differential role of fenretinide and all-trans RA in regulating the death and survival of human hepatocellular carcinoma Huh7 cells., PMID:23396089

Amphibian oocytes release heat shock protein A during spawning: evidence for a role in fertilization., PMID:22623622

The effects of fever on hormone ghrelins, immunoglobulins, and heat shock protein 70 expression after swine flu vaccinations., PMID:22477150

Cellular protein quality control and the evolution of aggregates in spinocerebellar ataxia type 3 (SCA3)., PMID:21916928

Measuring Hsp72 (HSPA1A) by indirect sandwich ELISA., PMID:21898234

Variants of HSPA1A in combination with plasma Hsp70 and anti-Hsp70 antibody levels associated with higher risk of acute coronary syndrome., PMID:21849784

Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies., PMID:21165727

Comment to: Humanization of a mouse monoclonal antibody directed against a cell surface-exposed epitope of membrane-associated heat shock protein 70 (Hsp70)., PMID:20668964

Extracellular heat shock protein 70 (HSPA1A) and classical vascular risk factors in a general population., PMID:20490736

HspBP1 levels are elevated in breast tumor tissue and inversely related to tumor aggressiveness., PMID:18987994

The equatorial subsegment in mammalian spermatozoa is enriched in tyrosine phosphorylated proteins., PMID:18448843

Association analysis of heat shock protein 70 gene polymorphisms in schizophrenia., PMID:18299791

Comparison of anti-heat shock protein 70 (anti-hsp70) and anti-68-kDa inner ear protein in the sera of patients with Meniere's disease., PMID:9023247

Pharmacological induction of heat shock protein 68 synthesis in cultured rat astrocytes., PMID:8530397

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-HSPA1A Polyclonal Antibody [PHC69301]
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