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Anti-ALOX5 Polyclonal Antibody

Catalog #:   PHC44201 Specific References (15) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P09917
Overview

Catalog No.

PHC44201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ALOX5 (Glu461-Ser671).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

5-LO,ALOX5,LOG5,Polyunsaturated fatty acid 5-lipoxygenase,5-lipoxygenase,Arachidonate 5-lipoxygenase

Purification

Purified by antigen affinity column.

Accession

P09917

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ALOX5 antibody (PHC44201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
References

Xin-Yi-Qing-Fei-Tang and its critical components reduce asthma symptoms by suppressing GM-CSF and COX-2 expression in RBL-2H3 cells., PMID:38631485

Immunohistochemical analysis of arachidonate 5-lipoxygenase expression in B-cell lymphomas: Implication for B cell differentiation and its analogy with lymphomagenesis., PMID:36689839

Evaluation of You-Gui-Wan critical compounds inhibiting ALOX-5 and HDC gene expression in RBL-2H3 cells using a fractional factorial design., PMID:36610671

Analysis of proximal ALOX5 promoter binding proteins by quantitative proteomics., PMID:32096311

Frontline Science: A reduction in DHA-derived mediators in male obesity contributes toward defects in select B cell subsets and circulating antibody., PMID:30576001

Biosynthesis of proresolving lipid mediators by vascular cells and tissues., PMID:28442547

Comparative Expression Analyses of Pro- versus Anti-Inflammatory Mediators within Synovium of Patients with Joint Trauma, Osteoarthritis, and Rheumatoid Arthritis., PMID:28316377

Lipid mediators foster the differentiation of T follicular helper cells., PMID:27838468

Genetic Predictors of Drug Hypersensitivity., PMID:27779086

Genetic variants associated with drugs-induced immediate hypersensitivity reactions: a PRISMA-compliant systematic review., PMID:26678823

Tocilizumab attenuates inflammation in ALS patients through inhibition of IL6 receptor signaling., PMID:23383400

Cyclooxygenase-2 generates the endogenous mutagen trans-4-hydroxy-2-nonenal in Enterococcus faecalis-infected macrophages., PMID:23321929

A novel function of IL-2: chemokine/chemoattractant/retention receptor genes induction in Th subsets for skin and lung inflammation., PMID:22464450

Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help., PMID:21224059

ALOX5 promoter genotype and response to montelukast in moderate persistent asthma., PMID:18339529

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-ALOX5 Polyclonal Antibody [PHC44201]
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