Catalog No.
YHK56101
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Arg72-His457
Predicted molecular weight
45.14 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9Y697
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
NFS1, NIFS, Cysteine desulfurase, mitochondrial
Identification of a Selective Inhibitor of Human NFS1, a Cysteine Desulfurase Involved in Fe-S Cluster Assembly, via Structure-Based Virtual Screening., PMID:40141425
Hippo pathway activation mediates cardiomyocyte ferroptosis to promote dilated cardiomyopathy through downregulating NFS1., PMID:40107016
Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation., PMID:39970777
Frataxin Traps Low Abundance Quaternary Structure to Stimulate Human Fe-S Cluster Biosynthesis., PMID:39909887
GLS1-mediated glutamine metabolism mitigates oxidative stress-induced matrix degradation, ferroptosis, and senescence in nucleus pulposus cells by modulating Fe2+ homeostasis., PMID:39710108
Two-stage binding of mitochondrial ferredoxin-2 to the core iron-sulfur cluster assembly complex., PMID:39632806
Integration of epigenomic and transcriptomic profiling uncovers EZH2 target genes linked to cysteine metabolism in hepatocellular carcinoma., PMID:39516467
Engineering a xylose fermenting yeast for lignocellulosic ethanol production., PMID:39496815
Impaired Iron-Sulfur Cluster Synthesis Induces Mitochondrial PARthanatos in Diabetic Cardiomyopathy., PMID:39495652
NFS1 inhibits ferroptosis in gastric cancer by regulating the STAT3 pathway., PMID:39254861
NFS1 as a Candidate Prognostic Biomarker for Gastric Cancer Correlated with Immune Infiltrates., PMID:39253726
Mitochondrial acyl carrier protein, Acp1, required for iron-sulfur cluster assembly in mitochondria and cytoplasm in Saccharomyces cerevisiae., PMID:39251117
Acute inhibition of iron-sulfur cluster biosynthesis disrupts metabolic flexibility in mice., PMID:39229169
Characterizing mitochondrial features in osteoarthritis through integrative multi-omics and machine learning analysis., PMID:39026663
Mapping Novel Frataxin Mitochondrial Networks Through Protein- Protein Interactions., PMID:38746130
Near-infrared imaging for visualizing the synergistic relationship between autophagy and NFS1 protein during multidrug resistance using an ICT-TICT integrated platform., PMID:38665516
Mechanism and structural dynamics of sulfur transfer during de novo [2Fe-2S] cluster assembly on ISCU2., PMID:38627381
PLA inhibits TNF-α-induced PANoptosis of prostate cancer cells through metabolic reprogramming., PMID:38408537
A Bimetallic Polymerization Network for Effective Increase in Labile Iron Pool and Robust Activation of cGAS/STING Induces Ferroptosis-Based Tumor Immunotherapy., PMID:38072786
Resveratrol drives ferroptosis of acute myeloid leukemia cells through Hsa-miR-335-5p/NFS1/ GPX4 pathway in a ROS-dependent manner., PMID:37715395
Direct Cysteine Desulfurase Activity Determination by NMR and the Study of the Functional Role of Key Structural Elements of Human NFS1., PMID:37410592
Pioglitazone treatment increases the cellular acid-labile and protein-bound sulfane sulfur fractions., PMID:37285721
Hydrogen sulfide alleviates mitochondrial damage and ferroptosis by regulating OPA3-NFS1 axis in doxorubicin-induced cardiotoxicity., PMID:36924813
Essential mitochondrial role in iron-sulfur cluster assembly of the cytoplasmic isopropylmalate isomerase Leu1 in Saccharomyces cerevisiae., PMID:36773733
The Human Mercaptopyruvate Sulfurtransferase TUM1 Is Involved in Moco Biosynthesis, Cytosolic tRNA Thiolation and Cellular Bioenergetics in Human Embryonic Kidney Cells., PMID:36671528
Selection of synthetic proteins to modulate the human frataxin function., PMID:36225115
Therapeutic approach with commercial supplements for pantothenate kinase-associated neurodegeneration with residual PANK2 expression levels., PMID:35945593
The ataxia-telangiectasia mutated gene product regulates the cellular acid-labile sulfide fraction., PMID:35696854
A fast responsive and cell membrane-targetable near-infrared H2S fluorescent probe for drug resistance bioassays in chemotherapy., PMID:35678466
Phosphorylated NFS1 weakens oxaliplatin-based chemosensitivity of colorectal cancer by preventing PANoptosis., PMID:35221331
Analyzing large scale gene expression data in colorectal cancer reveals important clues; CLCA1 and SELENBP1 downregulated in CRC not in normal and not in adenoma., PMID:35141024
During FeS cluster biogenesis, ferredoxin and frataxin use overlapping binding sites on yeast cysteine desulfurase Nfs1., PMID:35026224
Expanding the phenotypic and molecular spectrum of NFS1-related disorders that cause functional deficiencies in mitochondrial and cytosolic iron-sulfur cluster containing enzymes., PMID:35026043
N-terminal tyrosine of ISCU2 triggers [2Fe-2S] cluster synthesis by ISCU2 dimerization., PMID:34824239
Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites., PMID:34612205
Iron-sulphur cluster biogenesis factor LYRM4 is a novel prognostic biomarker associated with immune infiltrates in hepatocellular carcinoma., PMID:34488769
Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors., PMID:34452919
The Arabidopsis Iron-Sulfur (Fe-S) Cluster Gene MFDX1 Plays a Role in Host and Nonhost Disease Resistance by Accumulation of Defense-Related Metabolites., PMID:34281196
Molecular Details of the Frataxin-Scaffold Interaction during Mitochondrial Fe-S Cluster Assembly., PMID:34199378
Mammalian mitochondrial iron-sulfur cluster biogenesis and transfer and related human diseases., PMID:37288145
A recurring NFS1 pathogenic variant causes a mitochondrial disorder with variable intra-familial patient outcomes., PMID:33457206
Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a Genome Integrity Liability that Can Be Exploited by Targeting DNA Polymerase ε., PMID:33152268
Iron-Sulfur Cluster Protein NITROGEN FIXATION S-LIKE1 and Its Interactor FRATAXIN Function in Plant Immunity., PMID:32943465
Frataxin Structure and Function., PMID:31939159
Mammalian iron-sulfur cluster biogenesis: Recent insights into the roles of frataxin, acyl carrier protein and ATPase-mediated transfer to recipient proteins., PMID:31918395
Structure of the Human ACP-ISD11 Heterodimer., PMID:31664822
Mechanism of activation of the human cysteine desulfurase complex by frataxin., PMID:31511419
Physiologically relevant reconstitution of iron-sulfur cluster biosynthesis uncovers persulfide-processing functions of ferredoxin-2 and frataxin., PMID:31395877
Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism., PMID:31101807
Altered levels of mitochondrial NFS1 affect cellular Fe and S contents in plants., PMID:31065779
For research use only. Not for human or drug use.
