Catalog No.
PHC21201
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human ENO1 (Pro267-Leu354).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Non-neural enolase,2-phospho-D-glycerate hydro-lyase,MPB-1,ENO1,Plasminogen-binding protein,Enolase 1,C-myc promoter-binding protein,Alpha-enolase,MBP-1,MBPB1,MPB1,ENO1L1,NNE,Phosphopyruvate hydratase
Purification
Purified by antigen affinity column.
Accession
P06733
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Application of serum anti-ENO1 and anti-SSNA1 antibody biomarkers in predicting the prognosis of gastric cancer., PMID:40469915
Epitopes targeted by autoantibodies in presymptomatic individuals predict early rheumatoid arthritis., PMID:40348635
Machine learning technique-based four-autoantibody test for early detection of esophageal squamous cell carcinoma: a multicenter, retrospective study with a nested case-control study., PMID:40264204
Potential Novel Tissue Biomarkers in Salivary Glands of Patients with Sjogren's Syndrome., PMID:40217840
Immunohistochemical Analysis of Inter-Alpha-Trypsin Inhibitor Heavy Chain 2 and Enolase 1 in Canine Mammary Tumors: Associations with Tumor Aggressiveness and Prognostic Significance., PMID:40005870
Comprehensive Characterization of Anti-HLA and Non-HLA Antibodies in Patients on Kidney Transplant Waiting List and Evaluation of Their Impact on Alloimmunization Risk and Dialysis Treatment., PMID:39596170
O-GlcNAcylation of enolase 1 serves as a dual regulator of aerobic glycolysis and immune evasion in colorectal cancer., PMID:39446384
PI3Kγ inhibition combined with DNA vaccination unleashes a B-cell-dependent antitumor immunity that hampers pancreatic cancer., PMID:38824552
The Role of α-Enolase on the Production of Interleukin (IL)-32 in Con A-Mediated Inflammation and Rheumatoid Arthritis (RA)., PMID:38675491
Candida albicans cDNA library screening reveals novel potential diagnostic targets for invasive candidiasis., PMID:38657353
A critical view on autoantibodies in lupus nephritis: Concrete knowledge based on evidence., PMID:38552995
Anti-ENO1 antibody combined with metformin against tumor resistance: a novel antibody-based platform., PMID:38515460
Identification of host protein ENO1 (alpha-enolase) interacting with Cryptosporidium parvum sporozoite surface protein, Cpgp40., PMID:38504274
Characterization of chicken-derived antibody against Alpha-Enolase of Streptococcus pneumoniae., PMID:38185035
Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis., PMID:37964270
Circulating autoantibodies to alpha-enolase (ENO1) and far upstream element-binding protein 1 (FUBP1) are negative prognostic factors for pancreatic cancer patient survival., PMID:37910256
Unrevealed roles of extracellular enolase‑1 (ENO1) in promoting glycolysis and pro‑cancer activities in multiple myeloma via hypoxia‑inducible factor 1α., PMID:37800625
Enolase 1 of Candida albicans binds human CD4+ T cells and modulates naïve and memory responses., PMID:37503840
Vascular and Non-HLA autoantibody profiles in hospitalized patients with COVID-19., PMID:37398658
Generation and characterization of avian single chain variable fragment against human Alpha-Enolase., PMID:37196558
The efficacy and adverse effects of nivolumab and lenvatinib in the treatment of advanced hepatocellular carcinoma., PMID:37114307
Determination of the Predictive Roles and Potentially Pathogenic Antigen Epitopes of α-Enolase Related to the Development of Miscarriage in Females with Autoimmune Thyroiditis., PMID:36674531
[Relationship between anti-ENO1 antibody and systemic lupus erythematosus patients with retinopathy]., PMID:36533339
ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC., PMID:36361568
The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring., PMID:36149061
A Predictive Role of Autoantibodies Against the Epitope aa168-183 of ENO1 in the Occurrence of Miscarriage Related to Thyroid Autoimmunity., PMID:35707546
A Novel Enolase-1 Antibody Targets Multiple Interacting Players in the Tumor Microenvironment of Advanced Prostate Cancer., PMID:35700013
Expression of ENO1 Is Up-regulated in Low-grade Glioma and Positively Correlated With Meningioma Grade., PMID:35489746
An auto-antibody identified from phenotypic directed screening platform shows host immunity against EV-A71 infection., PMID:35130884
Nivolumab plus chemotherapy versus placebo plus chemotherapy in patients with HER2-negative, untreated, unresectable advanced or recurrent gastric or gastro-oesophageal junction cancer (ATTRACTION-4): a randomised, multicentre, double-blind, placebo-controlled, phase 3 trial., PMID:35030335
The proteomes of endometrial stromal cell-derived extracellular vesicles following a decidualizing stimulus define the cells' potential for decidualization success., PMID:34524461
Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis., PMID:34201883
Alpha-enolase involvement in intestinal and extraintestinal manifestations of celiac disease., PMID:34189450
ENO1 Promotes Lung Cancer Metastasis via HGFR and WNT Signaling-Driven Epithelial-to-Mesenchymal Transition., PMID:34145039
New insight to the rol of α-enolase (Eno-1) as immunological marker in rainbow trout fry., PMID:34118278
ENO1 monoclonal antibody inhibits invasion, proliferation and clone formation of cervical cancer cells., PMID:34094663
Aspirin modulates 2-hydroxyisobutyrylation of ENO1K281 to attenuate the glycolysis and proliferation of hepatoma cells., PMID:34000466
Diagnostic value of anti-citrullinated α-enolase peptide 1 antibody in patients with rheumatoid arthritis: A systematic review and meta-analysis., PMID:33713557
Alpha-enolase (ENO1), identified as an antigen to monoclonal antibody 12C7, promotes the self-renewal and malignant phenotype of lung cancer stem cells by AMPK/mTOR pathway., PMID:33579362
Proteomics-based analysis indicating α-enolase as a potential biomarker in primary Sjögren's syndrome., PMID:33447556
Serum IgG2 antibody multicomposition in systemic lupus erythematosus and lupus nephritis (Part 1): cross-sectional analysis., PMID:33374003
Serum IgG2 antibody multi-composition in systemic lupus erythematosus and in lupus nephritis (Part 2): prospective study., PMID:33351137
Multi-Autoantibody Signature and Clinical Outcome in Membranous Nephropathy., PMID:33257410
Early dose reduction/delay and the efficacy of liposomal irinotecan with fluorouracil and leucovorin in metastatic pancreatic ductal adenocarcinoma (mPDAC): A post hoc analysis of NAPOLI-1., PMID:33214082
In pancreatic cancer, chemotherapy increases antitumor responses to tumor-associated antigens and potentiates DNA vaccination., PMID:33115943
Preventing Candida albicans from subverting host plasminogen for invasive infection treatment., PMID:33115324
α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis., PMID:32934720
Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy., PMID:32442899
Generation and Characterization of Single Chain Variable Fragment against Alpha-Enolase of Candida albicans., PMID:32326294
ENO1-targeted superparamagnetic iron oxide nanoparticles for detecting pancreatic cancer by magnetic resonance imaging., PMID:32285549