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Anti-CKM Polyclonal Antibody

Catalog #:   PHC21101 Specific References (46) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P06732
Overview

Catalog No.

PHC21101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CKM (Met1-Lys381).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Creatine kinase M chain,Creatine phosphokinase M-type,Creatine kinase M-type,CKMM,CKM,CPK-M,M-CK

Purification

Purified by antigen affinity column.

Accession

P06732

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with CKM antibody (PHC21101) at 0.5μg/ml.

    Lane 1: Mouse heart lysate
    Lane 2: Mouse rectus abdominis muscle lysate
    Lane 3: Rat heart lysate
    Lane 4: Rat cerebrum lysate
    Lane 5: Rat skeletal muscle lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 44 kDa
    Observed MW: 44 kDa
  • Immunocytochemistry/ Immunofluorescence
    CKM in NIH3T3 Cell Line.
    The NIH3T3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CKM (PHC21101) at 10 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CKM antibody (PHC21101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 45 kDa
    Observed MW: 45 kDa
References

Molecular and Functional Significance of Growth Differentiation Factor-15: A Review on Cardiovascular-Kidney-Metabolic Biomarker., PMID:39781722

Talquetamab plus Teclistamab in Relapsed or Refractory Multiple Myeloma., PMID:39778168

Ocular Surface Evaluation in Immunoglobulin G4-Related Ophthalmic Disease., PMID:37544494

Isatuximab, carfilzomib, and dexamethasone in patients with relapsed multiple myeloma: updated results from IKEMA, a randomized Phase 3 study., PMID:37156782

Covid-19 a triggering factor of autoimmune and multi-inflammatory diseases., PMID:36858313

Disturbance of suprachiasmatic nucleus function improves cardiac repair after myocardial infarction by IGF2-mediated macrophage transition., PMID:36747104

Evaluation of the theoretical risk of cross-reactivity among recently identified food allergens for dogs., PMID:36043337

A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model., PMID:35688851

Population Immunity and Covid-19 Severity with Omicron Variant in South Africa., PMID:35196424

Human immune reactivity of GGTA1/CMAH/A3GALT2 triple knockout Yucatan miniature pigs., PMID:34232440

Subcutaneous daratumumab in Asian patients with heavily pretreated multiple myeloma: subgroup analyses of the noninferiority, phase 3 COLUMBA study., PMID:33599794

Daratumumab, bortezomib, and dexamethasone in relapsed or refractory multiple myeloma: subgroup analysis of CASTOR based on cytogenetic risk., PMID:32819447

Proteomic Analysis of Uterine Tissues During Peri-Implantation Period in Mice with Experimentally Induced Adenomyosis that Treated with anti-Ngf: Implications for Cell-Cell Adhesion and Metabolic Processes., PMID:32676925

Generation of an alpaca-derived nanobody recognizing γ-H2AX., PMID:26500838

Atypical neurological complications of ipilimumab therapy in patients with metastatic melanoma., PMID:24482447

Regulation of mitochondrial oxidative metabolism by tumor suppressor FLCN., PMID:23150719

Immune activation resulting from NKG2D/ligand interaction promotes atherosclerosis., PMID:22104546

High levels of brain-type creatine kinase activity in human platelets and leukocytes: a genetic anomaly with autosomal dominant inheritance., PMID:22088263

Immunoglobulin profiles in a murine intermediate host model of resistance for Echinococcus granulosus infection., PMID:12911524

Production of recombinant human creatine kinase (r-hCK) isozymes by tandem repeat expression of M and B genes and characterization of r-hCK-MB., PMID:11238299

Putative creatine kinase M-isoform in human sperm is identifiedas the 70-kilodalton heat shock protein HspA2., PMID:10952940

An immunochemical mass assay for the direct measurement of creatine kinase MB2., PMID:7605108

An immunoinhibition assay for determination of creatine kinase isoforms in serum., PMID:8086523

Nonspecific stimulation of host defense by Corynebacterium kutscheri. III. Enhanced cytokine induction by the active moiety of C. kutscheri., PMID:1611281

Immunohistochemical study of childhood rhabdomyosarcomas and related neoplasms. Results of an Intergroup Rhabdomyosarcoma study project., PMID:1710539

[Clinical significance of creatine kinase isoform assay by a new reagent based on immunoinhibition method]., PMID:2051618

Spatial distribution of "tissue-specific" antigens in the developing human heart and skeletal muscle. I. An immunohistochemical analysis of creatine kinase isoenzyme expression patterns., PMID:2240609

Immunological determination of the oligomeric form of mitochondrial creatine kinase in situ., PMID:2335207

A two-tube immunochemical method for determination of CK-MB isoenzyme in serum evaluated., PMID:2178803

Monoclonal antibody inhibiting creatine kinase MM3 but not isoform MM1., PMID:2297910

Creatine kinase isozyme expression in prenatal rat heart., PMID:2244691

IgA-CK-BB complex with CK-MB electrophoretic mobility can lead to erroneous diagnosis of acute myocardial infarction., PMID:2776341

[The determination of creatine kinase isoenzyme subunits CK-M and CK-B with the help of precipitating antibodies]., PMID:2711729

Creatine kinase-inhibiting monoclonal antibodies: preparation and characterization of porcine MM isoenzyme-specific antibodies., PMID:3173359

Effect of creatine kinase-MM subtype composition on a CK-MB immunoinhibition assay., PMID:3349605

Mitochondrial creatine kinase in cancer patients., PMID:2456506

Clinical significance and characteristics of creatine kinase-immunoglobulin complexes in sera from patients with malignant tumors., PMID:3095003

Electrophoretic characteristics of macro creatine kinase type 2 in serum., PMID:3940693

Demonstration of myoglobin and CK-M in myocardium. Comparison of five fixation methods and three immunohistochemical techniques., PMID:3902962

Macro creatine kinase type 1 with electrophoretic mobility identical to that of the MB isoenzyme., PMID:3893798

Apparent mitochondrial creatine kinase in the serum of a patient with metastatic cancer to the liver., PMID:7438444

Observation of a variant creatine kinase isoenzyme in sera and breast tumor cytosols., PMID:7416084

Mitochondrial CK (EC 2.7.3.2) in the human heart., PMID:6987009

[The use of primate tissue homogenates for quality control in the creatine kinase-MB determination with inhibiting antibodies (author's transl)]., PMID:408459

Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Activity kinetics and diagnostic significance in myocardial infarction., PMID:1000863

[Isoenzymes of creatine kinase in extracardiac disease and after various diagnostic and therapeutic procedures]., PMID:1278031

Datasheet

Document Download

Anti-CKM Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-CKM Polyclonal Antibody [PHC21101]
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