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Anti-APOA4 Polyclonal Antibody

Catalog #:   PHC20701 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P06727
Overview

Catalog No.

PHC20701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human APOA4 (Glu21-Ser396).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Apolipoprotein A-IV,ApoA-IV,Apo-AIV,APOA4,Apolipoprotein A4

Purification

Purified by antigen affinity column.

Accession

P06727

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with APOA4 antibody (PHC20701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 46 kDa
    Observed MW: 46 kDa
  • Immunocytochemistry/ Immunofluorescence
    APOA4 in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with APOA4 (PHC20701) at 7.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Subcutaneous Adipose Tissue-Secreted Proteins as Endocrine Regulators of Physical and Cognitive Function in Older Adults., PMID:40502786

Apolipoprotein A-IV regulates coagulation and ischemic stroke by potentiating activated protein C., PMID:40499720

Miniaturized 3D-printed ratiometric electrochemical immunosensing platform for ultrasensitive detection of depression biomarker Apo-A4., PMID:39566154

Distinct protein patterns related to postnatal development in small for gestational age preterm infants., PMID:39152333

APOA4 as a novel predictor of prognosis in Stevens-Johnson syndrome/toxic epidermal necrolysis: A proteomics analysis from two prospective cohorts., PMID:36963506

Investigating immunosensor for determination of depression marker-Apo-A4 based on patterning AuNPs and N-Gr nanomaterials onto ITO-PET flexible electrodes with amplifying signal., PMID:35998995

Identification of potential plasma protein biomarkers for feline pancreatic carcinoma by liquid chromatography tandem mass spectrometry., PMID:35514180

A stepwise data interpretation process for renal amyloidosis typing by LMD-MS., PMID:35418036

Proximity hybridization induced rolling circle amplification for label-free SERS detection of the depression marker human apolipoprotein A4., PMID:35398633

Hyperlipidemic plasma molecules bind and inhibit adiponectin activity., PMID:35023319

Low-density lipoprotein receptor-related protein 1 (LRP1) is a novel receptor for apolipoprotein A4 (APOA4) in adipose tissue., PMID:34168225

Label-free electrochemical homogeneous detection of the depression marker human apolipoprotein A4 based on proximity hybridization triggered rolling circle amplification., PMID:34111486

Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer., PMID:33602229

The New and the Old: Platform Cross-Validation of Immunoaffinity MASS Spectrometry versus ELISA for PromarkerD, a Predictive Test for Diabetic Kidney Disease., PMID:33126588

A novel electrochemical immunosensor for the highly sensitive and selective detection of the depression marker human apolipoprotein A4., PMID:32388438

Histological, immunohistochemical and mRNA gene expression responses in coeliac disease patients challenged with gluten using PAXgene fixed paraffin-embedded duodenal biopsies., PMID:31730447

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease., PMID:31618575

Apolipoprotein Profiles in Very Preterm and Term-Born Preschool Children., PMID:30968745

Expression analysis of apolipoproteins AI & AIV in hepatocellular carcinoma: A protein-based hepatocellular carcinoma-associated study., PMID:29998871

Evaluation of candidate genes associated with hepatitis A and E virus infection in Chinese Han population., PMID:29558945

[Classification of cardiac amyloidosis: an immunohistochemical analysis]., PMID:29429161

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease., PMID:28803590

Clinical, biopsy, and mass spectrometry findings of renal gelsolin amyloidosis., PMID:28139293

Clinical, biopsy, and mass spectrometry characteristics of renal apolipoprotein A-IV amyloidosis., PMID:27262366

Expression and oxidative modifications of plasma proteins in autism spectrum disorders: Interplay between inflammatory response and lipid peroxidation., PMID:27246309

Identification of haptoglobin peptide as a novel serum biomarker for lung squamous cell carcinoma by serum proteome and peptidome profiling., PMID:26783151

Phosphorylation and SCF-mediated degradation regulate CREB-H transcription of metabolic targets., PMID:26108621

Specific expression of apolipoprotein A-IV in the follicle-associated epithelium of the small intestine., PMID:24838500

Clinical diagnosis and typing of systemic amyloidosis in subcutaneous fat aspirates by mass spectrometry-based proteomics., PMID:24747948

The effect of colostrum intake on blood plasma proteome profile in newborn lambs: low abundance proteins., PMID:24708841

Renal amyloidosis: origin and clinicopathologic correlations of 474 recent cases., PMID:23704299

Apolipoprotein A-IV as a novel gene associated with polycystic ovary syndrome., PMID:23338533

Proteomic identification of serum biomarkers for gastric cancer using multi-dimensional liquid chromatography and 2D differential gel electrophoresis., PMID:22446497

[Study on proteomic specificity of unstable angina with qi deficiency and blood stasis syndrome]., PMID:19702077

Kininogen in autoimmune uveitis: decrease in peripheral blood stream versus increase in target tissue., PMID:19696180

Afamin and apolipoprotein A-IV: novel protein markers for ovarian cancer., PMID:19336561

New serological biomarkers of inflammatory bowel disease., PMID:18777587

Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis., PMID:18061280

[Monitoring changes of serum protein markers in metastatic colorectal carcinoma model]., PMID:17374239

Effect of immunoaffinity depletion of human serum during proteomic investigations., PMID:16212426

Immunohistochemical localization of apolipoprotein A-IV in human kidney tissue., PMID:16105043

The role of apolipoprotein AIV on the control of food intake., PMID:15777185

Human apolipoprotein A-IV reduces secretion of proinflammatory cytokines and atherosclerotic effects of a chronic infection mimicked by lipopolysaccharide., PMID:14751811

Structure-function studies of human apolipoprotein A-V: a regulator of plasma lipid homeostasis., PMID:12899628

Plasma distribution of apoA-IV in patients with coronary artery disease and healthy controls., PMID:12777472

Quantitative measurement of lipoprotein particles containing both apolipoprotein AIV and apolipoprotein B in human plasma by a noncompetitive ELISA., PMID:12029004

Disruption of hedgehog signaling reveals a novel role in intestinal morphogenesis and intestinal-specific lipid metabolism in mice., PMID:11832461

Effect of leptin on intestinal apolipoprotein AIV in response to lipid feeding., PMID:11506989

Antioxidative and antiatherosclerotic effects of human apolipoprotein A-IV in apolipoprotein E-deficient mice., PMID:11397714

Intestinal satiety protein apolipoprotein AIV is synthesized and regulated in rat hypothalamus., PMID:11294757

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-APOA4 Polyclonal Antibody [PHC20701]
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