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Anti-BCHE Polyclonal Antibody

Catalog #:   PHC19401 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P06276
Overview

Catalog No.

PHC19401

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BCHE (Glu29-Thr150).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Choline esterase II,CHE1,Cholinesterase,BCHE,Butyrylcholine esterase,Acylcholine acylhydrolase,Pseudocholinesterase

Purification

Purified by antigen affinity column.

Accession

P06276

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with BCHE antibody (PHC19401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with BCHE antibody (PHC19401) at 1 µg/ml.

    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Raji cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 68 kDa
    Observed MW: 70 kDa
References

Investigating biomarkers of exposure to jet aircraft oil fumes using mass spectrometry., PMID:40313268

Low levels of endogenous cholinesterases support the choice of cows, sheep and goats for the transgenic expression of human butyrylcholinesterase in milk., PMID:37659623

Butyrylcholinesterase-Protein Interactions in Human Serum., PMID:34639003

Organophosphate detoxification by membrane-engineered red blood cells., PMID:33529769

A Method for Diagnosing Organophosphate Pesticide Exposure in Humans using Liquid Chromatography Coupled Tandem Mass Spectrometry., PMID:33367644

Aerobic Barley Mg-protoporphyrin IX Monomethyl Ester Cyclase is Powered by Electrons from Ferredoxin., PMID:32911631

Monoclonal antibodies to fetal bovine serum acetylcholinesterase distinguish between acetylcholinesterases from ruminant and non-ruminant species., PMID:32795450

Novel cholinesterase paralogs of Schistosoma mansoni have perceived roles in cholinergic signalling and drug detoxification and are essential for parasite survival., PMID:31809524

Development of a long-acting Fc-fused cocaine hydrolase with improved yield of protein expression., PMID:30986387

Mass spectrometry based proteomic approach for the screening of butyrylcholinesterase adduct formation with organophosphates., PMID:30771950

Characterization of butyrylcholinesterase from porcine milk., PMID:29908755

A Nanozyme- and Ambient Light-Based Smartphone Platform for Simultaneous Detection of Dual Biomarkers from Exposure to Organophosphorus Pesticides., PMID:29792679

Delipidation of Plasma Has Minimal Effects on Human Butyrylcholinesterase., PMID:29497381

Simultaneous detection of dual biomarkers from humans exposed to organophosphorus pesticides by combination of immunochromatographic test strip and ellman assay., PMID:29306031

Comparison of Efficiency of Purification (from Human Plasma) of a Nerve Agent Adduct of Butyrylcholinesterase Between the Affinity Gel Method and Immunomagnetic Separation., PMID:29244127

Monoclonal Antibody That Recognizes Diethoxyphosphotyrosine-Modified Proteins and Peptides Independent of Surrounding Amino Acids., PMID:29137457

Hupresin Retains Binding Capacity for Butyrylcholinesterase and Acetylcholinesterase after Sanitation with Sodium Hydroxide., PMID:29066970

Oxidative stress in dairy cows seropositives for Neospora caninum., PMID:28915999

The C5 Variant of the Butyrylcholinesterase Tetramer Includes a Noncovalently Bound 60 kDa Lamellipodin Fragment., PMID:28661448

Anticeramide antibody and butyrylcholinesterase in peripheral neuropathies., PMID:28576432

High-Confidence Qualitative Identification of Organophosphorus Nerve Agent Adducts to Human Butyrylcholinesterase., PMID:28208252

Acetylcholinesterase Regulates Skeletal In Ovo Development of Chicken Limbs by ACh-Dependent and -Independent Mechanisms., PMID:27574787

Cholinergic enzymes and inflammatory markers in rats infected by Sporothrix schenckii., PMID:27260685

Monoclonal antibodies to human butyrylcholinesterase reactive with butyrylcholinesterase in animal plasma., PMID:26585590

Pharmacokinetics and immunogenicity of a recombinant human butyrylcholinesterase bioscavenger in macaques following intravenous and pulmonary delivery., PMID:26415620

Immunohistochemical analysis of hippocampal butyrylcholinesterase: Implications for regional vulnerability in Alzheimer's disease., PMID:26293308

Pure human butyrylcholinesterase hydrolyzes octanoyl ghrelin to desacyl ghrelin., PMID:26073531

Detection of cresyl phosphate-modified butyrylcholinesterase in human plasma for chemical exposure associated with aerotoxic syndrome., PMID:24892986

An enhanced butyrylcholinesterase method to measure organophosphorus nerve agent exposure in humans., PMID:24604326

Direct quantitation of methyl phosphonate adducts to human serum butyrylcholinesterase by immunomagnetic-UHPLC-MS/MS., PMID:24205842

Experimental treatments for cocaine toxicity: a difficult transition to the bedside., PMID:23978563

Magnetic Fe3O4@TiO2 nanoparticles-based test strip immunosensing device for rapid detection of phosphorylated butyrylcholinesterase., PMID:23911770

Gene transfer of mutant mouse cholinesterase provides high lifetime expression and reduced cocaine responses with no evident toxicity., PMID:23840704

Effect of polyethylene glycol conjugation on the circulatory stability of plasma-derived human butyrylcholinesterase in mice., PMID:23220586

Preparation, characterization of Fe3O4 at TiO2 magnetic nanoparticles and their application for immunoassay of biomarker of exposure to organophosphorus pesticides., PMID:23122753

Monoclonal antibodies to mouse butyrylcholinesterase., PMID:23099085

Combined cocaine hydrolase gene transfer and anti-cocaine vaccine synergistically block cocaine-induced locomotion., PMID:22912888

Identification of human cytochrome P450 isoforms and esterases involved in the metabolism of mirabegron, a potent and selective β3-adrenoceptor agonist., PMID:22509825

Identification of phosphorylated butyrylcholinesterase in human plasma using immunoaffinity purification and mass spectrometry., PMID:22444575

Characterization of human serum butyrylcholinesterase in rhesus monkeys: behavioral and physiological effects., PMID:22402122

Modification of pharmacokinetic and abuse-related effects of cocaine by human-derived cocaine hydrolase in monkeys., PMID:22264200

Interception of cocaine by enzyme or antibody delivered with viral gene transfer: a novel strategy for preventing relapse in recovering drug users., PMID:22229308

Enzyme-linked immunosorbent assay for detection of organophosphorylated butyrylcholinesterase: a biomarker of exposure to organophosphate agents., PMID:21504805

Magnetic electrochemical sensing platform for biomonitoring of exposure to organophosphorus pesticides and nerve agents based on simultaneous measurement of total enzyme amount and enzyme activity., PMID:21462919

A novel system for the efficient generation of antibodies following immunization of unique knockout mouse strains., PMID:20886120

Structure-activity relationship of quaternary acetylcholinesterase inhibitors - outlook for early myasthenia gravis treatment., PMID:20345342

Demonstration of in vivo stability and lack of immunogenicity of a polyethyleneglycol-conjugated recombinant CHO-derived butyrylcholinesterase bioscavenger using a homologous macaque model., PMID:20211615

Pharmacokinetics and immunologic consequences of repeated administrations of purified heterologous and homologous butyrylcholinesterase in mice., PMID:19772863

Adenovirus-transduced human butyrylcholinesterase in mouse blood functions as a bioscavenger of chemical warfare nerve agents., PMID:19542320

Neurotoxic and immunotoxic effects of Indole-3-butyric acid on rats at subacute and subchronic exposure., PMID:19442822

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-BCHE Polyclonal Antibody [PHC19401]
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