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Anti-EDN1/Endothelin1 Polyclonal Antibody

Catalog #:   PHC15901 Specific References (20) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P05305
Overview

Catalog No.

PHC15901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EDN1 (Ala18-Trp212).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Endothelin-1,EDN1,PPET1,ET-1,Preproendothelin-1,Endothelin1

Purification

Purified by antigen affinity column.

Accession

P05305

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with EDN1 antibody (PHC15901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
  • Immunocytochemistry/ Immunofluorescence
    EDN1 in HaCat Cell Line.
    The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with EDN1 (PHC15901) at 8.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

VEPTP inhibition with an extracellular domain targeting antibody did not restore albuminuria in a mouse model of diabetic kidney disease., PMID:39324545

Comprehensive analysis of the endothelin system in the kidneys of mice, rats, and humans., PMID:38904098

Pharmacogenomics of intravenous immunoglobulin response in Kawasaki disease., PMID:38259468

Targeting IL-11 system as a treatment of pulmonary arterial hypertension., PMID:37949331

Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells., PMID:35899934

The Influence of Adalimumab and Cyclosporine A on the Expression Profile of the Genes Related to TGFβ Signaling Pathways in Keratinocyte Cells Treated with Lipopolysaccharide A., PMID:32774143

Identification of NFAT5 as a transcriptional regulator of the EDN1 gene in collecting duct., PMID:30623723

Intercellular Genetic Interaction Between Irf6 and Twist1 during Craniofacial Development., PMID:28769044

Cardiac fibroblast transcriptome analyses support a role for interferogenic, profibrotic, and inflammatory genes in anti-SSA/Ro-associated congenital heart block., PMID:28626076

Endothelin-1 genetic polymorphism as predictive marker for bevacizumab in metastatic breast cancer., PMID:27139155

MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells., PMID:24632479

Polymorphisms of endothelin 1 (G5665T and T-1370G) and endothelin receptor type A (C+70G and G-231A) in Graves' disease., PMID:24291390

The fate of renal allografts hinges on responses of the microvascular endothelium., PMID:22710034

Endothelin-1 is elevated in Alzheimer's disease and upregulated by amyloid-β., PMID:22330820

MTHFR and MSX1 contribute to the risk of nonsyndromic cleft lip/palate., PMID:20572854

Endothelin-1, endothelin converting enzyme-1 and endothelin receptors in the porcine corpus luteum., PMID:19783117

Transcriptome analyses in normal prostate epithelial cells exposed to low-dose cadmium: oncogenic and immunomodulations involving the action of tumor necrosis factor., PMID:18560533

Endothelin axis polymorphisms in patients with scleroderma., PMID:16947775

Variants of endothelin-1 and its receptors in atopic asthma., PMID:10448102

Aortic arch malformations and ventricular septal defect in mice deficient in endothelin-1., PMID:7615798

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-EDN1/Endothelin1 Polyclonal Antibody [PHC15901]
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