Catalog No.
YGK31701
Expression system
E. coli
Species
General
Protein length
Met1-Ala221
Predicted molecular weight
27.32 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P03772
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Flag tag Control Protein, Flag tag Protein, Flag-tag Control Protein, Flag-tag Protein
Identification of autoantibodies targeting citrullinated CLEC12A in rheumatoid arthritis patients., PMID:40336986
Epitope tags are not created equal: Disruption of cellular function of a translation factor by a short viral tag., PMID:40046037
Fluorescent tagging of endogenous IRS2 with an auxin-dependent degron to assess dynamic intracellular localization and function., PMID:39305958
Comprehensive posttranslational modifications in the testis-specific histone variant H3t protein validated in tagged knock-in mice., PMID:39266663
Expression mapping of GREM1 and functional contribution of its secreting cells in the brain using transgenic mouse models., PMID:38072150
Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIP., PMID:37738118
CUB domains are not required for OVCH2 function in sperm maturation in the mouse epididymis., PMID:37551853
Production of Plant-Derived Japanese Encephalitis Virus Multi-Epitope Peptide in Nicotiana benthamiana and Immunological Response in Mice., PMID:37511402
Improving Single-Molecule Antibody Detection Selectivity through Optimization of Peptide Epitope Presentation in OmpG Nanopore., PMID:37379512
Toxicity of C9orf72-associated dipeptide repeat peptides is modified by commonly used protein tags., PMID:37308278
Development of a 5-HT7 receptor antibody for the rat: the good, the bad, and the ugly., PMID:37071157
Epitope-tag-mediated synaptogenic activity in an engineered neurexin-1β lacking the binding interface with neuroligin-1., PMID:37030069
Effect of the Enhanced Production of Chlorophyll b on the Light Acclimation of Tomato., PMID:36834789
Insertion of exogenous genes within the ORF1b coding region of porcine astrovirus., PMID:36812864
Generation of two CRISPR/Cas edited human induced pluripotent stem cell lines (DHMi005-A-3 and DHMi005-A-4) carrying a FLAG-tag after exon 9 of the TBX5 gene., PMID:36610218
Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803., PMID:35969224
Different fusion tags affect the activity of ubiquitin overexpression on spastin protein stability., PMID:34873900
Tandem Affinity Purification (TAP) of Low-Abundance Protein Complexes in Filamentous Fungi Demonstrated Using Magnaporthe oryzae., PMID:34236680
The Scleraxis Transcription Factor Directly Regulates Multiple Distinct Molecular and Cellular Processes During Early Tendon Cell Differentiation., PMID:34150754
Glucose-Mediated Protein Arginine Phosphorylation/Dephosphorylation Regulates ylxR Encoding Nucleoid-Associated Protein and Cell Growth in Bacillus subtilis., PMID:33101263
Comparison of Efficiency and Function of Vascular Endothelial Growth Factor Adenovirus Vectors in Endothelial Cells for Gene Therapy of Placental Insufficiency., PMID:32988220
Molecular characterization of Arabidopsis thaliana LSH1 and LSH2 genes., PMID:32803702
Transcriptome-Wide Mapping of Protein-RNA Interactions., PMID:32681512
Source-sink modifications affect leaf senescence and grain mass in wheat as revealed by proteomic analysis., PMID:32503423
Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification., PMID:32478734
Human GDPD3 overexpression promotes liver steatosis by increasing lysophosphatidic acid production and fatty acid uptake., PMID:32430316
Rapid Generation of Attenuated Infectious Bursal Disease Virus from Dual-Promoter Plasmids by Reduction of Viral Ribonucleoprotein Activity., PMID:31915284
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations., PMID:31762451
Sodium butyrate-activated TRAF6-TXNIP pathway affects A549 cells proliferation and migration., PMID:31578830
TAG-RNAi overcomes off-target effects in cancer models., PMID:31558799
Alternative translation initiation generates the N-terminal truncated form of RUNX1 that retains hematopoietic activity., PMID:30690039
Modulating the Expression Strength of the Baculovirus/Insect Cell Expression System: A Toolbox Applied to the Human Tumor Suppressor SMARCB1/SNF5., PMID:30178299
Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae., PMID:30120352
Unexpected link between insect innexins and apoptosis of HeLa cells., PMID:29862562
A membrane-type surfactant protein D (SP-D) suppresses macrophage-mediated cytotoxicity in swine endothelial cells., PMID:29425774
SIX1 and DACH1 influence the proliferation and apoptosis of hepatocellular carcinoma through regulating p53., PMID:29333942
Progress toward an enhanced vaccine: Eight marked attenuated viruses to porcine reproductive and respiratory disease virus., PMID:29324359
Safe Staphylococcal Platform for the Development of Multivalent Nanoscale Vesicles against Viral Infections., PMID:29253342
5-Azacytidine treatment and TaPBF-D over-expression increases glutenin accumulation within the wheat grain by hypomethylating the Glu-1 promoters., PMID:29214328
Nisin-induced expression of recombinant T cell epitopes of major Japanese cedar pollen allergens in Lactococcus lactis., PMID:29094185
Secretory expression and purification of Bacillus licheniformis keratinase in insect cells., PMID:28832667
Identification and characterization of a Golgi retention signal in feline coronavirus accessory protein 7b., PMID:28758629
TFE3 regulates renal adenocarcinoma cell proliferation via activation of the mTOR pathway., PMID:28713889
Chicken RNA-binding protein T-cell internal antigen-1 contributes to stress granule formation in chicken cells and tissues., PMID:28693298
Organic anion transporting polypeptide 1B3 can form homo- and hetero-oligomers., PMID:28644885
Functional characterizations and expression profiles of ADAR2 gene, responsible for RNA editing, in response to GCRV challenge in grass carp (Ctenopharyngodon idella)., PMID:27514783
Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture., PMID:26965256
SPAK and OSR1 Sensitive Cell Membrane Protein Abundance and Activity of KCNQ1/E1 K+ Channels., PMID:26584301
A novel nonsense mutation in the MIP gene linked to congenital posterior polar cataracts in a Chinese family., PMID:25803033
Generation of plasmid vectors expressing FLAG-tagged proteins under the regulation of human elongation factor-1α promoter using Gibson assembly., PMID:25742071
For research use only. Not for human or drug use.
