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Anti-Human CSF2/GM-CSF Antibody (SAA0418)

Catalog #:   FHC06710 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2a, kappa
Applications: ELISA, FCM
Accession: P04141
Overview

Catalog No.

FHC06710

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

Granulocyte-macrophage colony-stimulating factor, Sargramostim, GMCSF, CSF, Colony-stimulating factor, CSF2, GM-CSF, Molgramostin

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P04141

Applications

ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.09% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0418

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human CSF2 antibody.CSF2 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # FHC06710 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human CSF2 antibody.HL-60 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # FHC06710 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

Adaptive immune changes in colorectal cancer: a focus on T and B cell activation genes., PMID:40484866

Lower expression of colony-stimulating factor 2, an embryokine, in the endometrial epithelium of old cows., PMID:39951370

Cytokine Autoantibodies Alter Gene Expression Profiles of Healthy Donors., PMID:39551979

In vitro analysis of VEGF-mediated endothelial permeability and the potential therapeutic role of Anti-VEGF in severe dengue., PMID:39263317

Amplification of autoimmune organ damage by NKp46-activated ILC1s., PMID:39137897

PAR2 activation on human tubular epithelial cells engages converging signaling pathways to induce an inflammatory and fibrotic milieu., PMID:39005931

The mechanism of multistep progression of the transcriptional cascade in activated microglia as approached by a proteome approach., PMID:38824862

Cell-Based Models of 'Cytokine Release Syndrome' Endorse CD40L and Granulocyte-Macrophage Colony-Stimulating Factor Knockout in Chimeric Antigen Receptor T Cells as Mitigation Strategy., PMID:37947658

The RNA binding proteins ZFP36L1 and ZFP36L2 are dysregulated in airway epithelium in human and a murine model of asthma., PMID:37928904

PAK1 overexpression promotes myxofibrosarcoma angiogenesis through STAT5B-mediated CSF2 transactivation: clinical and therapeutic relevance of amplification and nuclear entry., PMID:37564209

Identification of new immune subtypes of renal injury associated with anti-neutrophil cytoplasmic antibody-associated vasculitis based on integrated bioinformatics analysis., PMID:37091784

Utility of atherosclerosis-associated serum antibodies against colony-stimulating factor 2 in predicting the onset of acute ischemic stroke and prognosis of colorectal cancer., PMID:36844744

Pleiotropic effects of pitavastatin: a pilot study using the saphenous vein endothelial cell model of endothelial injury and prevention of atherosclerosis., PMID:35916819

Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation., PMID:35563458

CSF2-dependent monocyte education in the pathogenesis of ANCA-induced glomerulonephritis., PMID:35418479

Pancreatic Ductal Adenocarcinoma (PDAC) circulating tumor cells influence myeloid cell differentiation to support their survival and immunoresistance in portal vein circulation., PMID:35316296

CD73 induces GM-CSF/MDSC-mediated suppression of T cells to accelerate pancreatic cancer pathogenesis., PMID:35001076

Subunit-Specific Reactivity of Autoantibodies Against Laminin-332 Reveals Direct Inflammatory Mechanisms on Keratinocytes., PMID:34899732

Neutralizing Anti-Granulocyte-Macrophage Colony-Stimulating Factor Autoantibodies in Patients With Central Nervous System and Localized Cryptococcosis: Longitudinal Follow-up and Literature Review., PMID:34718451

Robust innate responses to SARS-CoV-2 in children resolve faster than in adults without compromising adaptive immunity., PMID:34587479

Glucocorticoids and Androgens Protect From Gastric Metaplasia by Suppressing Group 2 Innate Lymphoid Cell Activation., PMID:33971182

Granulocyte-Macrophage colony stimulating factor in asthmatic patients infected with respiratory syncytial virus., PMID:33742625

Platelet activation contributes to hypoxia-induced inflammation., PMID:33264579

Novel cytokine-antibody fusion protein, N-820, to enhance the functions of ex vivo expanded natural killer cells against Burkitt lymphoma., PMID:33109629

[Inflammatory mechanism of hippocampal tissue injury induced by PM2.5 in nasal drip in mice]., PMID:32981279

Csf2 Attenuated Sepsis-Induced Acute Kidney Injury by Promoting Alternative Macrophage Transition., PMID:32733471

Amphiregulin Aggravates Glomerulonephritis via Recruitment and Activation of Myeloid Cells., PMID:32616537

Tumour-derived CSF2/granulocyte macrophage colony stimulating factor controls myeloid cell accumulation and progression of gliomas., PMID:32390004

Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells., PMID:31743940

Pan-viral serology implicates enteroviruses in acute flaccid myelitis., PMID:31636453

Immunoglobulin-driven Complement Activation Regulates Proinflammatory Remodeling in Pulmonary Hypertension., PMID:31545648

Interleukin-15 Enhances Anti-GD2 Antibody-Mediated Cytotoxicity in an Orthotopic PDX Model of Neuroblastoma., PMID:31455682

BLT-Immune Humanized Mice as a Model for Nivolumab-Induced Immune-Mediated Adverse Events: Comparison of the NOG and NOG-EXL Strains., PMID:30850839

Sanshool improves UVB-induced skin photodamage by targeting JAK2/STAT3-dependent autophagy., PMID:30622245

Pharmacodynamic biomarkers and differential effects of TNF- and GM-CSF-targeting biologics in rheumatoid arthritis., PMID:30358109

Involvement and Possible Role of Eosinophils in Asthma Exacerbation., PMID:30323811

Expansion of Interleukin-22- and Granulocyte-Macrophage Colony-Stimulating Factor-Expressing, but Not Interleukin-17A-Expressing, Group 3 Innate Lymphoid Cells in the Inflamed Joints of Patients With Spondyloarthritis., PMID:30260078

Association Between Plasma Level of Collagen Type III Alpha 1 Chain and Development of Strictures in Pediatric Patients With Crohn's Disease., PMID:30213581

Murine models of Pneumocystis infection recapitulate human primary immune disorders., PMID:29925696

IL-3 but not monomeric IgE regulates FcεRI levels and cell survival in primary human basophils., PMID:29724998

GM-CSF and IL-4 are not involved in IVIG-mediated amelioration of ITP in mice: a role for IL-11 cannot be ruled out., PMID:29704458

Adjuvant Effect of Bacille Calmette-Guérin on Hepatitis B Vaccine Immunogenicity in the Preterm and Term Newborn., PMID:29416539

Effects of total flavonoids of sea buckthorn ( Hippophae rhamnoides L.) on cytotoxicity of NK92-MI cells., PMID:28994628

Cortisol inhibits CSF2 and CSF3 via DNA methylation and inhibits invasion in first-trimester trophoblast cells., PMID:28846166

Mechanistic Sharing Between NK Cells in ABMR and Effector T Cells in TCMR., PMID:28654216

Class IIa HDAC inhibition reduces breast tumours and metastases through anti-tumour macrophages., PMID:28273064

Elderly-onset hereditary pulmonary alveolar proteinosis and its cytokine profile., PMID:28212655

Evidence for CD16a-Mediated NK Cell Stimulation in Antibody-Mediated Kidney Transplant Rejection., PMID:27906829

Depletion of CD4+ CD25+ regulatory T cells confers susceptibility to experimental autoimmune encephalomyelitis (EAE) in GM-CSF-deficient Csf2-/- mice., PMID:27256565

Candidate early detection protein biomarkers for ER+/PR+ invasive ductal breast carcinoma identified using pre-clinical plasma from the WHI observational study., PMID:26319120

Datasheet
$ 198
Product specifications
50 μg 198 100 μg 328

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Anti-Human CSF2/GM-CSF Antibody (SAA0418) [FHC06710]
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