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Anti-Human IL10 Antibody (SAA0378)

Catalog #:   FHD50710 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1, kappa
Applications: Blocking, ELISA, FCM
Accession: P22301
Overview

Catalog No.

FHD50710

Species reactivity

Human

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

IL-10, IL10, Cytokine synthesis inhibitory factor, CSIF, Interleukin-10

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P22301

Applications

Blocking, ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.09% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0378

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human IL10 antibody.IL10 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL10 antibody monoclonal antibody (Catalog # FHD50710 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with IL10 antibody (FHD50710) at 1 µg/ml.

    Lane 1: IL10 transfected HEK293 cell lysate
    Lane 2: Non-transfected HEK293 cell lysate

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
References

Stereotactic body radiation therapy (SBRT) increases anti-PD-1 antitumor activity by enhancing the tumor immune microenvironment in mice with metastatic hepatocellular carcinoma., PMID:40512454

A Mixture of Free and Microencapsulated Essential Oils Combined with Turmeric and Tannin in the Diet of Dairy Cows: Effects on Productive Efficiency and Animal Health., PMID:40509055

Anti-Tumor Activities of Anti-Siglec-15 Chimeric Heavy-Chain Antibodies., PMID:40507880

Profile of Cytokines Associated with SARS-CoV2 Seropositivity in Multiple Sclerosis Patients and Its Persistence over Six Months., PMID:40507498

Effects of Rhodobacter sphaeroides protein on growth performance, nutrient digestibility, rumen fermentation, and serum metabolites in dairy calves during the pre- and postweaning periods., PMID:40499903

Selected cytokine and chemokine concentrations in equine autologous conditioned serum are similar under defined and practically relevant storage conditions., PMID:40496923

miR-125a-5p Regulates Treg Function by Targeting Foxp3 in Experimental Autoimmune Myasthenia Gravis Mice., PMID:40490083

The role of cytokines in pediatric hematologic malignancies: mechanisms of tumor progression and therapeutic implications - a narrative review., PMID:40486628

Adaptive immune changes in colorectal cancer: a focus on T and B cell activation genes., PMID:40484866

Enterococcus faecalis hijacks FABP2 to activate quorum-sensing signals and aggravate Crohn's disease by inducing gut dysbiosis., PMID:40484632

Advances of oncolytic vaccinia viruses armed with interleukin in tumor therapy., PMID:40469178

Longitudinal analysis of cytokine dynamics in severe fever with thrombocytopenia syndrome patients - High-incidence regions of China (2010-2023)., PMID:40453472

Circulating immunoregulatory B cell and autoreactive antibody profiles predict lack of toxicity to anti-PD-1 checkpoint inhibitor treatment in advanced melanoma., PMID:40449958

Differences in Aqueous Humor Cytokine Concentrations According to the Presence of Posterior Subcapsular Cataract: A Prospective Case-Control Study., PMID:40441712

Potential of bone marrow mesenchymal stem cells in iodine-induced autoimmune thyroiditis therapy., PMID:40435196

Immunological biomarkers and their role in the diagnosis and prognosis of leishmaniasis: A case-control study., PMID:40433642

Recombinant C5a Peptidase and Formalin-Killed Cell: A Synergistic Vaccine Against Streptococcus iniae in Four-Finger Threadfin Fish (Eleutheronema tetradactylum)., PMID:40420522

[Protective аction of ginseng root extract on myofibril apoptosis and immune response in rats after exhausting physical exercise. Part II. Effect of ginseng root extract on immunity and regulation of energy metabolism]., PMID:40418135

CD19+IL-10+ regulatory B cells induced by IL-12p35 regulates functional T-cell subsets in patients with immune thrombocytopenia., PMID:40415408

Investigating Anti-Tumor Effects of Irisin in Cervical Cancer Cells: Cell Viability, Migration, and Tumor-Associated Macrophage Polarization., PMID:40415369

Live attenuated hfq-deleted Acinetobacter baumannii vaccine provides protection against systemic infection., PMID:40412336

Evaluation of humoral and cellular immune responses in healthcare workers with varying levels of SARS-CoV-2 exposure: effects of CoronaVac vaccination followed by heterologous booster., PMID:40406109

Clinical, genetic, and proteomic characteristics of type 2 diabetes complicated with exogenous insulin antibody syndrome: a case-control study., PMID:40404055

Effects of infliximab infusion on clinical symptom scores and serum cytokines in patients with inflammatory bowel disease., PMID:40400059

Development of tolerogenic casein encapsulated quercetin and curcumin nanoparticles to mitigate cow milk allergic responses., PMID:40398766

Association of neurobiological and immune serum biomarkers with Toxoplasma gondii infection in patients with schizophrenia., PMID:40397143

Systemic secukinumab treatment in patients with ankylosing spondylitis: the relationship between systemic and tear proinflammatory cytokines and ocular surface findings-a pilot study., PMID:40394377

The MVA-VP2-NS1-2A-NS2-Nt vaccine candidate provides heterologous protection in sheep against bluetongue virus., PMID:40391210

IFNγ Signaling Impairs Regulatory B Cell Function Resulting in Worse Control of Esophageal Food Allergy., PMID:40387177

Plasminogen deficiency reduces disease severity and immune responses in enterovirus A71-infected mice., PMID:40377310

Effects of novel sulfated-rhamnoglucuronan isolated from Korean seaweed Ulva pertusa on IgA-mediated intestinal-immune system activation in naïve C3H/HeN mice., PMID:40373895

Effects of dietary supplementation with bile acids on growth performance, antioxidant capacity, lipid metabolism, and cecal microbiota of Danzhou chickens., PMID:40373624

Сomprehensive approach to comparative characteristics of clinical and laboratory parameters of the study in children - in 6 months after Covid-19 treatment and 6 months after Covid-19 vaccination., PMID:40367469

Regulatory T cells suppress TLR9-induced formation of intrahepatic myeloid-cell aggregates for T cell population expansion in liver., PMID:40366446

Tamdy virus pathogenesis in immunocompetent and immunocompromised mouse models., PMID:40354169

Relation of Cytokine Profile and Antibody Values to Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis (Preliminary Data)., PMID:40353964

Unveiling the immune and vitamin profiles of blood: the potential biomarkers for alopecia areata., PMID:40352276

Xevinapant plus avelumab in advanced solid tumours, with a dose expansion in advanced non-small-cell lung cancer: exploratory biomarker, safety and efficacy analyses from an open-label, nonrandomised phase Ib study., PMID:40351326

Bifidobacterium longum BL300 alleviates DSS-induced Colitis by promoting IgA-mediated Gut Microbiota homeostasis., PMID:40350789

Regulatory cytokines modulate early isotype-specific response associated with COVID-19 survival., PMID:40342417

Phenotype and function of IL-10-producing NK cells in individuals with malaria experience., PMID:40337867

PROFILES FOR SOME KEY CYTOKINES AND AUTO-ANTIBODIES IN LATENT TOXOPLASMOSIS IN ABORTED WOMEN., PMID:40335055

Oral Delivery of Lactococcus lactis Expressing Full-Length S Protein via Alginate-Chitosan Capsules Induces Immune Protection Against PEDV Infection in Mice., PMID:40333306

Pregnant Women Chronically Infected by Toxoplasma gondii with Depressive Disorder: Differential Modulation of Pro-Inflammatory and Anti-Inflammatory Cytokines., PMID:40333139

JEG-3 Trophoblast Cells Influence ILC-like Transformation of NK Cells In Vitro., PMID:40332223

Allergen Immunotherapy: Pitfalls, Perks and Unexpected Allies., PMID:40332034

Correlation among ocular surface changes and systemic hematologic indexes and disease activity in primary Sjögren's syndrome: a cross-sectional study., PMID:40329232

Understanding the impact of moxifloxacin on immune function: Findings from cytokine analyses and immunological assays in mice., PMID:40327662

Combination therapy with cetirizine and anti-PD-1 antibody suppresses colitis-induced colon tumor formation in mice., PMID:40320111

Secretory IgA binding to FCRL3 triggers shared inflammatory cytokine secretion by human regulatory T cells and effector T cells., PMID:40313182

Datasheet
$ 198
Product specifications
50 μg 198 100 μg 328

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Anti-Human IL10 Antibody (SAA0378) [FHD50710]
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