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Anti-PGK1 Polyclonal Antibody

Catalog #:   PHB87101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P00558
Overview

Catalog No.

PHB87101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PGK1 (Ser2-Ile417).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PRP 2,Primer recognition protein 2,PGKA,PGK1,Phosphoglycerate kinase 1,Cell migration-inducing gene 10 protein

Purification

Purified by antigen affinity column.

Accession

P00558

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with PGK1 antibody (PHB87101) at 0.42μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: HuH-7 cell lysate
    Lane 3: 293T cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 44 kDa
    Observed MW: 44 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PGK1 antibody (PHB87101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 47 kDa
    Observed MW: 47 kDa
References

An Exosomal mRNA Urine Test for Detection and Risk Stratification of Human Kidney Transplant Rejection., PMID:40303229

Anti-PGK1 antibodies in immuno-related pancytopenia., PMID:40008495

From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different Candida albicans Extracellular Vesicles., PMID:39796100

Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes., PMID:37189694

Identification and evaluation of novel serum autoantibody biomarkers for early diagnosis of gastric cancer and precancerous lesion., PMID:37079049

Quantitative Acetylomics Revealed Acetylation-Mediated Molecular Pathway Network Changes in Human Nonfunctional Pituitary Neuroendocrine Tumors., PMID:34712204

The proteomes of endometrial stromal cell-derived extracellular vesicles following a decidualizing stimulus define the cells' potential for decidualization success., PMID:34524461

A Universal Labeling Strategy for Nucleic Acids in Expansion Microscopy., PMID:34424689

The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma., PMID:34221965

Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis., PMID:34201883

Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection., PMID:34200478

Simultaneous Isolation of Circulating Nucleic Acids and EV-Associated Protein Biomarkers From Unprocessed Plasma Using an AC Electrokinetics-Based Platform., PMID:33224932

Associations of PGK1 promoter hypomethylation and PGK1-mediated PDHK1 phosphorylation with cancer stage and prognosis: a TCGA pan-cancer analysis., PMID:31578148

Multi-dimensional immunoproteomics coupled with in vitro recapitulation of oncogenic NRASQ61R identifies diagnostically relevant autoantibody biomarkers in thyroid neoplasia., PMID:31326556

Autoantibody-targeted TAAs in pancreatic cancer: A comprehensive analysis., PMID:31255446

Energy metabolism is co-determined by genetic variants in chronic lymphocytic leukemia and influences drug sensitivity., PMID:30792207

PGK1 as an immune target in Kawasaki disease., PMID:30113070

Using a Commonly Down-Regulated Cytomegalovirus (CMV) Promoter for High-Level Expression of Ectopic Gene in a Human B Lymphoma Cell Line., PMID:29244783

A probe-based qRT-PCR method to profile immunological gene expression in blood of captive beluga whales (Delphinapterus leucas)., PMID:28970970

Screening novel autoantigens targeted by serum IgG autoantibodies in immunorelated pancytopenia by SEREX., PMID:28687989

Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody., PMID:26865113

Candida albicans Shaving to Profile Human Serum Proteins on Hyphal Surface., PMID:26696967

Characteristics of testis-specific phosphoglycerate kinase 2 and its association with human sperm quality., PMID:26677959

Serological response and diagnostic value of recombinant candida cell wall protein enolase, phosphoglycerate kinase, and β-glucosidase., PMID:26441862

An antibody reactivity-based assay for diagnosis of invasive candidiasis using protein array., PMID:25280031

Der p 2 can induce bystander activation of B cells derived from patients with systemic lupus erythematosus., PMID:25130611

[Prokaryotic expression and immunogenicity analysis of phosphoglycerate kinase 1 of Candida albicans]., PMID:24103269

Proteomic identification of radiation response markers in mouse intestine and brain., PMID:21319302

The identification of auto-antibodies in pancreatic cancer patient sera using a naturally fractionated Panc-1 cell line., PMID:21045262

Expression of Trichoderma reesei cellulases CBHI and EGI in Ashbya gossypii., PMID:20422178

Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzymes., PMID:19284558

Synthetic glycopeptide vaccines combining beta-mannan and peptide epitopes induce protection against candidiasis., PMID:18725625

A sandwich ELISA for phosphoglycerate kinase., PMID:18569371

Clonality of the parathyroid nodules with uremic parathyroid hyperplasia., PMID:18470730

Immunoglobulin G responses to a panel of Candida albicans antigens as accurate and early markers for the presence of systemic candidiasis., PMID:18322056

Monoclonal anti-double-stranded DNA antibodies cross-react with phosphoglycerate kinase 1 and inhibit the expression and production of IL-2 in activated Jurkat T cell line., PMID:16857429

Proteomic study reveals that proteins involved in metabolic and detoxification pathways are highly expressed in HER-2/neu-positive breast cancer., PMID:16048908

Ex vivo localization of the mouse saposin C activation region for acid beta-glucosidase., PMID:12126932

Comparative expression and purification of human glutamic acid decarboxylase from Saccharomyces cerevisiae and Pichia pastoris., PMID:10862868

Continuous delivery of human and mouse erythropoietin in mice by genetically engineered polymer encapsulated myoblasts., PMID:10326023

Distinct multiple RET/PTC gene rearrangements in multifocal papillary thyroid neoplasia., PMID:9814501

Expression in Saccharomyces cerevisiae of antigenically and enzymatically active recombinant glutamic acid decarboxylase., PMID:9650286

The presence of a Sar1 gene family in Brassica campestris that suppresses a yeast vesicular transport mutation Sec12-1., PMID:9154984

3-phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of Candida albicans., PMID:9043109

X-Inactivation and histone H4 acetylation in embryonic stem cells., PMID:8954732

Random primer p(dN)6-digoxigenin labeling for quantitation of mRNA by Q-RT-PCR and ELISA., PMID:7702858

Direct effect of basic fibroblast growth factor on gene transcription in a cell-free system., PMID:1535155

Clonal hematopoiesis in patients with acquired aplastic anemia., PMID:1683796

Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency., PMID:6604128

Comparison of the two purified allozymes (1B and 1A) of X-linked phosphoglycerate kinase in the mouse., PMID:6683503

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-PGK1 Polyclonal Antibody [PHB87101]
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