Catalog No.
PHB85301
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human DHFR (Met1-Asp187).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Dihydrofolate reductase,DHFR
Purification
Purified by antigen affinity column.
Accession
P00374
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Genomic and Phenotypic Characterization of CHO 4BGD Cells with Quad Knockout and Overexpression of Two Housekeeping Genes That Allow for Metabolic Selection and Extended Fed-Batch Culturing., PMID:40422195
Development of a Dihydrofolate Reductase Selection System for Saccharomyces boulardii., PMID:40076696
Trypanosoma cruzi infection in American black bears (Ursus americanus): A case report in a cub from California and serologic survey for exposure in wild black bears from several states., PMID:39550185
Prodrug nanotherapy demonstrates in vivo anticryptosporidial efficacy in a mouse model of chronic Cryptosporidium infection., PMID:39372445
Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform., PMID:38801272
Targeted Drug Delivery by MMAE Farnesyl-Bioconjugated Multivalent Chemically Self-Assembled Nanorings Induces Potent Receptor-Dependent Immunogenic Cell Death., PMID:38701361
Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9., PMID:38651269
Sustained clinical benefit of malaria chemoprevention with sulfadoxine-pyrimethamine (SP) in pregnant women in a region with high SP resistance markers., PMID:38574776
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines., PMID:37985175
Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium., PMID:36562657
Use of Leishmania major parasites expressing a recombinant Trypanosoma cruzi antigen as live vaccines against Chagas disease., PMID:36523834
Hybrid cell line development system utilizing site-specific integration and methotrexate-mediated gene amplification in Chinese hamster ovary cells., PMID:36185448
Evaluation of site-specific methylation of the CMV promoter and its role in CHO cell productivity of a recombinant monoclonal antibody., PMID:35719211
QbD-guided pharmaceutical development of Pembrolizumab biosimilar candidate PSG-024 propelled to industry meeting primary requirements of comparability to Keytruda®., PMID:35378209
Enhancement of anti-TNFα monoclonal antibody production in CHO cells through the use of UCOE and DHFR elements in vector construction and the optimization of cell culture media., PMID:34427158
A proline metabolism selection system and its application to the engineering of lipid biosynthesis in Chinese hamster ovary cells., PMID:34386349
A phase 1 study of NY-ESO-1 vaccine + anti-CTLA4 antibody Ipilimumab (IPI) in patients with unresectable or metastatic melanoma., PMID:33796406
Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies., PMID:33314785
Expression of recombinant classical swine fever virus E2 glycoprotein by endogenous Txnip promoter in stable transgenic CHO cells., PMID:32774204
Construction of a Mammalian IRES-based Expression Vector to Amplify a Bispecific Antibody; Blinatumomab., PMID:32184875
Complexes Formed via Bioconjugation of Genetically Modified TMV Particles with Conserved Influenza Antigen: Synthesis and Characterization., PMID:32093598
Attenuated glutamine synthetase as a selection marker in CHO cells to efficiently isolate highly productive stable cells for the production of antibodies and other biologics., PMID:31043114
Incorporation of CD40 ligand or granulocyte-macrophage colony stimulating factor into Hantaan virus (HTNV) virus-like particles significantly enhances the long-term immunity potency against HTNV infection., PMID:30657443
Recombinant Antibody Production in CHO and NS0 Cells: Differences and Similarities., PMID:30499081
An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High-Level Monoclonal Antibody Producing CHO DG44 Cell Lines., PMID:30196505
Application of microRNA Targeted 3'UTRs to Repress DHFR Selection Marker Expression for Development of Recombinant Antibody Expressing CHO Cell Pools., PMID:29981248
Gene variants in the folate pathway are associated with increased levels of folate receptor autoantibodies., PMID:29732742
Using a Dihydrofolate Reductase-Based Strategy for Producing the Biosimilar Version of Pertuzumab in CHO-S Cells., PMID:29437532
Identification and functional characterization of type II toxin/antitoxin systems in Aggregatibacter actinomycetemcomitans., PMID:29319934
A novel enediyne-integrated antibody-drug conjugate shows promising antitumor efficacy against CD30+ lymphomas., PMID:29316337
Proteomic analysis of CHO cell lines producing high and low quantities of a recombinant antibody before and after selection with methotrexate., PMID:29137976
Optimized Selection Marker and CHO Host Cell Combinations for Generating High Monoclonal Antibody Producing Cell Lines., PMID:29090854
Incorporation of Phosphorylated Tyrosine into Proteins: In Vitro Translation and Study of Phosphorylated IκB-α and Its Interaction with NF-κB., PMID:28898075
Impact of Signal Peptides on Furin-2A Mediated Monoclonal Antibody Secretion in CHO Cells., PMID:28727292
Proteome Analysis of Rheumatoid Arthritis Gut Mucosa., PMID:27627584
Identification of regulatory motifs in the CHO genome for stable monoclonal antibody production., PMID:27544513
Disruption of the gene C12orf35 leads to increased productivities in recombinant CHO cell lines., PMID:27183150
Carboxypeptidase D is the only enzyme responsible for antibody C-terminal lysine cleavage in Chinese hamster ovary (CHO) cells., PMID:26989081
In Vivo Evaluation of Site-Specifically PEGylated Chemically Self-Assembled Protein Nanostructures., PMID:26985775
miR-192 suppresses leptomeningeal dissemination of medulloblastoma by modulating cell proliferation and anchoring through the regulation of DHFR, integrins, and CD47., PMID:26506238
Deep sequencing reveals different compositions of mRNA transcribed from the F8 gene in a panel of FVIII-producing CHO cell lines., PMID:25963793
A nanotherapy strategy significantly enhances anticryptosporidial activity of an inhibitor of bifunctional thymidylate synthase-dihydrofolate reductase from Cryptosporidium., PMID:25900220
Synthetic antibodies and peptides recognizing progressive multifocal leukoencephalopathy-specific point mutations in polyomavirus JC capsid viral protein 1., PMID:25879139
CEA/CD3 bispecific antibody MEDI-565/AMG 211 activation of T cells and subsequent killing of human tumors is independent of mutations commonly found in colorectal adenocarcinomas., PMID:25484061
Construction of a chimeric secretory IgA and its neutralization activity against avian influenza virus H5N1., PMID:24741594
[The expression of interferon-lambda1 in CHO cell]., PMID:24319953
Cell line profiling to improve monoclonal antibody production., PMID:24249214
Construction and characterization of a novel chimeric antibody c3C7 specific for the integrin αIIbβ3 complex., PMID:24113827
Transcriptome dynamics of transgene amplification in Chinese hamster ovary cells., PMID:24108600
Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihydrofolate reductase of Escherichia coli., PMID:23911607
For research use only. Not for human or drug use.
