Every researcher dreads two scenarios: being unable to find reliable protocols, or meticulously following steps only to get irreproducible results! Worry no more. These extensively tested protocol repositories cover everything from fundamental techniques to cutting-edge methods – offering free access, downloadable PDFs, and video tutorials. Your PI or lab mates might not have told you about these.
·Bio-protocol：Founded by a Stanford University team. Covers WB, qPCR, animal studies, cell culture, and more. All protocols are sourced from published papers. Free PDF downloads include reagent catalog number lists. Primary focus: Molecular Biology, Immunology, Developmental Biology, etc.
Web：https://bio-protocol.org/cn
·Protocol Exchange：An open platform by Nature. Covers 35 disciplines (from Cancer to Materials Science). Protocols are uploaded directly by authors, are free, citable (with DOI), and feature powerful filtering tools for efficiency.
Web：https://protocolexchange.researchsquare.com
·STAR Protocol: An open-access, peer-reviewed journal from Cell Press. Dedicated to transparent, reproducible methodologies across Life Sciences, Health Sciences, Earth Sciences, and Physical Sciences.
·JoVE（Journal of Visualized Experiments）: The world's only peer-reviewed video methods journal! Features videos recorded in top labs (Harvard, MIT, etc.), demonstrating procedures from tissue sectioning to animal surgery. Includes text protocols and material lists. Subscription required. Find select free videos via PubMed Central (PMC).
·Current Protocols: The most widely recognized experimental methods collection globally! Covers 19 disciplines with over 25,000 techniques. Includes critical troubleshooting guides and expected results.
Web：https://currentprotocols.onlinelibrary.wiley.com/
·Springer Nature Experiments: A major protocol database from Springer Nature. Hosts over 75,000 reproducible laboratory protocols spanning Molecular Biology, Genetics, Biochemistry, Molecular Medicine, and more.
·Cold Spring Harbor Protocols (CSH Protocols)：Curated by the renowned Cold Spring Harbor Laboratory (CSHL), this platform sets the gold standard for rigorously peer-reviewed, step-by-step experimental methodologies. It spans cutting-edge fields including molecular biology, genetics, neuroscience, and genomics.
